Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification
نویسندگان
چکیده
منابع مشابه
Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification
Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohy...
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Pectinase is used commercially in the clarification and extraction of fruit juice from different fruits. Green apples and Red apples obtained from the fruits section of a supermarket, Idiroko road, Ota were surface sterilized and inoculated with Aspergillus niger. The stock culture was subcultured on Sabouraud Dextrose agar plates and 72-hr-old culture of Aspergillus niger served as the inoculu...
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Characterization of a Polygalacturonase from Trichoderma harzianum Grown on Citrus Peel with Application for Apple Juice
A polygalacturonase (PGase) was purified from Trichoderma harzianum , grown on citrus peel, by anion exchange and size exclusion chromatographies. The enzyme had molecular mass of 29 kDa. The extent of hydrolysis by PGase was decreased with increasing of pectin esterification degree. m m ax K and V , using polygalacturonic acid as substrate, were 1.42 mg and 0.66 ìmol reducing sugar, respective...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2014
ISSN: 1517-8382
DOI: 10.1590/s1517-83822014000100014